Biotin (vitamin B7) is an essential micronutrient in animal feed, serving as a coenzyme for multiple carboxylases and playing a pivotal role in carbohydrate, fat, and protein metabolism. It is crucial for energy utilization, fatty acid synthesis, and nucleic acid synthesis. In practical livestock production, biotin exerts significant benefits across swine, poultry, ruminants, and aquatic species. For breeding pigs, it enhances hoof integrity, reduces lameness, and improves reproductive performance. In poultry, it promotes skin and feather development and supports hatchability. In ruminants, particularly dairy cows, biotin contributes to stronger hoof quality and may boost milk production. When included in aquaculture feeds, it stimulates growth and weight gain. Deficiencies can manifest as stunted growth, dermatitis, hair loss, cracked hooves, and reproductive disorders. Furthermore, the bioavailability of biotin can be compromised by raw egg white, oxidized rancid fats, and certain cereal grains in the diet, while gut diseases or prolonged medication may interfere with its endogenous synthesis. Therefore, prudent supplementation in feed formulations is required, with the dosage scientifically determined according to animal species and production stage.