Vitamin B2 (riboflavin) is an indispensable water-soluble vitamin in animal feed. As a core component of the coenzymes FMN and FAD, it is extensively involved in energy metabolism (particularly the oxidative breakdown of carbohydrates, fats, and proteins) and cellular respiration in the body. In livestock production, vitamin B2 is essential for maintaining normal growth, skin and mucosal health, visual function, and nervous system function. Deficiency can result in typical deficiency syndromes, such as "curled toe paralysis" in poultry, growth retardation, diarrhea, and ruffled feathers; dermatitis, eye disorders, and reproductive failure in pigs; and angular stomatitis in young ruminant calves. Since animals cannot synthesize it themselves, and its content in feed ingredients like grains is limited, especially under intensive production and high-performance demands, it is necessary to supplement it extra in complete feeds to meet the nutritional requirements for rapid growth, high egg production, and high reproductive performance.